rabbit polyclonal ym1 antibody  (STEMCELL Technologies Inc)

Journal: Cell Communication and Signaling : CCS

Article Title: NLRP3: a key regulator of skin wound healing and macrophage-fibroblast interactions in mice

doi: 10.1186/s12964-025-02063-9

Figure Lengend Snippet: Antibodies used in immunofluorescence staining

Article Snippet: Ym1 , Rabbit polyclonal , , 1:50 , Stemcell technologies , 60,130.

Techniques: Immunofluorescence

Journal: Cell Communication and Signaling : CCS

Article Title: NLRP3: a key regulator of skin wound healing and macrophage-fibroblast interactions in mice

doi: 10.1186/s12964-025-02063-9

Figure Lengend Snippet: Antibodies used in Western blot

Article Snippet: Ym1 , Rabbit polyclonal , , 1:50 , Stemcell technologies , 60,130.

Techniques: Western Blot, Concentration Assay

Journal: Diseases

Article Title: Dasatinib and Quercetin as Senolytic Drugs Improve Fat Deposition and Exhibit Antifibrotic Effects in the Medaka Metabolic Dysfunction-Associated Steatotic Liver Disease Model

doi: 10.3390/diseases12120317

Figure Lengend Snippet: Comparison of M1/M2 macrophages in liver tissues. ( A ) Immunohistochemistry staining for Ym1 and CD68. ( B ) The number of positive cells for Ym1 and CD68 was quantified. The Ym1-to-CD68 ratio was analyzed. Values are presented as means ± standard deviations. n = 13–20 per experiment. * p < 0.05.

Article Snippet: IHC was performed using rabbit anti-p21 polyclonal antibody (orb3599, Biorbyt, Cambridge, UK), rabbit anti-γH2AX polyclonal antibody (ab11174, Abcam, Cambridge, UK), rabbit anti-LMNB1 polyclonal antibody (ab16048, Abcam, Cambridge, UK), rabbit anti-Ym1 polyclonal antibody (#60130, STEMCELL technology, Vancouver, BC, Canada), rabbit anti-CD68 polyclonal antibody (ab125212, Abcam, Cambridge, UK), Vectastain Elite ABC Rabbit IgG kit (PK-6101; Vector Laboratories, Burlingame, CA, USA), and DAB chromogen tablet (Muto Pure Chemicals, Tokyo, Japan) for IHC.

Techniques: Comparison, Immunohistochemistry, Staining

Journal: mBio

Article Title: M2a macrophages facilitate resolution of chemically-induced colitis in TLR4-SNP mice

doi: 10.1128/mbio.01208-23

Figure Lengend Snippet: DSS-treated TLR4-SNP mice exhibit reduced M2a markers in the colon and reduced β-hydroxybutyrate (β-HB) levels in the sera. ( A ) M2a gene expression measured in the MC of male WT vs TLR4-SNP mice, comparing control, Day 9, and Day 11 samples by qRT-PCR. One-way ANOVA with Sidak multiple comparisons test. * P < 0.05; ** P < 0.01; **** P < 0.0001. Data are derived from two independent experiments. In total, control WT n = 11; control TLR4-SNP n = 15; WT Day 9 n = 13, Day 11 n = 13; TLR4-SNP Day 9 n = 15, Day 11 n = 15. ( B ) M2a (Ym1, Arg1) protein production measured in DC samples in WT vs TLR4-SNP mice, comparing control and Day 11 samples. Data are representative of results from two independent experiments totaling control WT n = 5; control TLR4-SNP n = 5; WT Day 11 n = 9; TLR4-SNP Day 11 n = 9. ( C ) M2a (Mrc1, PPARγ) protein production measured in DC samples in WT vs TLR4-SNP mice, comparing control and Day 11 samples. Data representative of results from two independent experiments totaling control WT n = 5; control TLR4-SNP n = 5; WT Day 11 n = 9; TLR4-SNP Day 11 n = 9. ( D ) β-HB concentration measured in the sera of control and DSS-treated, WT vs TLR4-SNP male mice at Days 9 and 11. Two-way ANOVA with Sidak multiple comparisons. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. Data are derived from three independent experiments in which, together, control WT n = 12; control TLR4-SNP n = 13; WT Day 11 n = 10; TLR4-SNP Day 11 n = 10.

Article Snippet: Primary anti-PPARγ rabbit polyclonal antibody (Abclonal A0270; 1:2,000 dilution), primary mouse monoclonal anti-Arginase 1 (BD Biosciences 610709; 1:1,000 dilution), primary rabbit polyclonal anti-Ym1 (StemCell 60130; 1:1,000 dilution), primary goat polyclonal anti-Mrc1 (R&D Systems AF2535-SP; 1:1,000 dilution), primary rabbit anti-GAPDH (14C10) monoclonal antibody (Cell Signaling 2118; 1:1,000 dilution), secondary peroxidase-conjugated goat anti-rabbit IgG antibody (Jackson ImmunoResearch 111-035-003; 1:5,000 dilution), secondary peroxidase-conjugated goat anti-mouse IgG light chain-specific antibody (Jackson ImmunoResearch 115-035-174; 1:5,000 dilution), and secondary peroxidase-conjugated rabbit anti-goat IgG antibody (Jackson ImmunoResearch 305-035-003; 1:5,000 dilution) were used to detect species-specific primary antibodies.

Techniques: Expressing, Quantitative RT-PCR, Derivative Assay, Concentration Assay

Journal: mBio

Article Title: M2a macrophages facilitate resolution of chemically-induced colitis in TLR4-SNP mice

doi: 10.1128/mbio.01208-23

Figure Lengend Snippet: Administration of rosiglitazone, a PPARγ agonist ligand, ameliorates DSS-induced colonic damage in TLR4-SNP mice. Male TLR4-SNP mice were administered DSS starting on Day 0. On Days 2–7, mice were administered either saline or rosiglitazone (25 mg/kg) once daily i.p. ( A ) Average colonic symptom score over time in saline- and rosiglitazone-treated male TLR4-SNP mice. Two-way ANOVA with Sidak’s multiple comparisons; * P < 0.05. ( B ) Colon lengths at Day 14 in saline- and rosiglitazone-treated TLR4-SNP mice. Unpaired two-tailed Students t- test. *** P < 0.001. ( C ) Representative H&E-stained images of DC sections from saline- and rosiglitazone-treated TLR4-SNP mice at Day 14. ( D ) High-power image of submucosal infiltrating inflammatory cells in a representative DSS- and saline-treated TLR4-SNP mouse on Day 14. The infiltrating cells are predominantly mononuclear with occasional granulocytic cells (red arrows). ( E ) Right panel: histology scores of H&E-stained DC sections from saline- and rosiglitazone-treated TLR4-SNP mice at Day 14. Unpaired two-tailed Students t- test; ** P < 0.01. Results were derived from three independent experiments. In total, TLR4-SNP saline n = 17; TLR4-SNP rosiglitazone n = 19. ( F ) Ym1 (Chil3) protein expression at Day 11 in DC of DSS-treated TLR4-SNP mice therapeutically administered rosiglitazone or saline. Data are representative of results from two independent experiments totaling TLR4-SNP rosiglitazone-treated n = 9; TLR4-SNP saline-treated n = 8.

Article Snippet: Primary anti-PPARγ rabbit polyclonal antibody (Abclonal A0270; 1:2,000 dilution), primary mouse monoclonal anti-Arginase 1 (BD Biosciences 610709; 1:1,000 dilution), primary rabbit polyclonal anti-Ym1 (StemCell 60130; 1:1,000 dilution), primary goat polyclonal anti-Mrc1 (R&D Systems AF2535-SP; 1:1,000 dilution), primary rabbit anti-GAPDH (14C10) monoclonal antibody (Cell Signaling 2118; 1:1,000 dilution), secondary peroxidase-conjugated goat anti-rabbit IgG antibody (Jackson ImmunoResearch 111-035-003; 1:5,000 dilution), secondary peroxidase-conjugated goat anti-mouse IgG light chain-specific antibody (Jackson ImmunoResearch 115-035-174; 1:5,000 dilution), and secondary peroxidase-conjugated rabbit anti-goat IgG antibody (Jackson ImmunoResearch 305-035-003; 1:5,000 dilution) were used to detect species-specific primary antibodies.

Techniques: Saline, Two Tailed Test, Staining, Derivative Assay, Expressing

Journal: The Journal of nutritional biochemistry

Article Title: A high docosahexaenoic acid diet alters lung inflammation and recovery following repetitive exposure to aqueous organic dust extracts

doi: 10.1016/j.jnutbio.2021.108797

Figure Lengend Snippet: Effect of high DHA diet and repetitive HDE exposure on the number of Ym1-positive cells in the lung at three weeks (A-C) and after the recovery period (D-F). Mice were fed high DHA or control diets for four weeks before commencing three-week repetitive 12.5% HDE exposure. Lung was fixed in formalin, and paraffin embedded sections were obtained for immunofluorescence staining of Ym1 and images were obtained on Echo Revolve epifluorescence microscope with 20X objective as described in . Lung representative images of Ym1+ immunofluorescence staining, number of Ym1+ macrophages and quantification of fluorescence intensity are shown on A, B and C for mice at three weeks after saline or HDE exposure, and on D, E and F for mice after the recovery period following 3-week repetitive HDE exposure. Number of Ym1+ cells were counted on ImageJ; n =5-7 for three weeks (B), and n =6 for the recovery period (E). Fluorescence intensity of the whole image was obtained by quantifying integrated density/area in Image J; n =4-6 for three weeks (C), and n =5-6 for the recovery period (F). Data are mean ± standard error of the mean. * P <.05, ** P <.01, *** P <.001, **** P <.0001.

Article Snippet: After the blocking step, tissues were incubated with rabbit polyclonal antibody against mouse Ym1 (Stem Cell Technologies, Cambridge, MA, USA, catalog #: 60130, 1:200 dilution), with rabbit antibody against mouse Arg-1 (Cell Signaling, MA, USA, catalog #: 93668, 1:50 dilution) and biotinylated GSL-1 (Vector Laboratories, Burlingame, CA, USA, catalog #: B-1205-.5, 1:50 dilution) in 1% BSA in PBST (0.1% Tween-20 in PBS) overnight at 4°C.

Techniques: Control, Immunofluorescence, Staining, Microscopy, Fluorescence, Saline

Journal: eLife

Article Title: CXCL10/CXCR3 signaling contributes to an inflammatory microenvironment and its blockade enhances progression of murine pancreatic precancerous lesions

doi: 10.7554/eLife.60646

Figure Lengend Snippet:

Article Snippet: Antibody , anti-YM1 (Rabbit polyclonal) , STEMCELL Technologies , Cat# 60130, RRID: AB_2868482 , IF (1:200).

Techniques: Control, RNAscope, In Situ Hybridization, Multiplex Assay, Recombinant, Sequencing, Software, Flow Cytometry, Cell Culture, Plasmid Preparation